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Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus.

Aparna, H. S. and Salimath, P. V. (1999) Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus. Nutrition Research, 19 (2). 295-303, 29 ref..

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Abstract

Acidic glycoproteins were isolated from buffalo colostrum at pH 4.6 and their yields were highest -in the first day colostrum, but decreased drastically in the third to fifth day samples. They were purified on DEAE-cellulose and CM-cellulose columns and exhibited complex nature. The concentrations of carbohydrates and proteins varied from 2.1-13.9 % and 80-96 % , respectively, on dry weight basis. The SDS-PAGE analysis showed the heterogeneous nature of the fractions and some of the bands also stained positive with periodate-Schiff’s reagent indicating their glycoprotein nature. The compositional analysis of the glycans revealed the presence of L-fucose, D-galactose, D-mannose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine and N-acetyl neuraminic acid in different proportions. Some of the isolated fractions were found to enhance the growth of Bifidobacterium bifidus var. Pennsylvanicus significantly at low concentrations.

Item Type: Article
Uncontrolled Keywords: Buffalo, Colostrum, Glycoproteins, Bzjidobacterium
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 27 Dairy products
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Feb 2012 08:56
Last Modified: 14 Feb 2012 08:56
URI: http://ir.cftri.com/id/eprint/2645

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