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A specific enzymatic procedure for the determination of neurotoxic components (derivatives of L-alpha,beta-diaminopropionic acid) in Lathyrus sativus.

Rao, D. R. and Hariharan, K. and Vijayalakshmi, K. R. (1974) A specific enzymatic procedure for the determination of neurotoxic components (derivatives of L-alpha,beta-diaminopropionic acid) in Lathyrus sativus. Journal of Agricultural and Food Chemistry, 22 (6). 1146-1148, 14 ref..

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Abstract

Lathyrus satiVU8 seeds are used to adulterate some of the common dietary pulses such as Cicer arietinum (Bengal gram) and Cajanus cajan (Red gram) in India despite food laws. Chromatographic or other procedures available for detection of the neurotoxic or other components in Lathyrus sotivus are nonspecific. In the present method, the neurotoxic components (derivatives of L-a.l3-diaminopropionic acid) are separated by cation exchange chromatography and subjected diamito acid hydrolysis. The L-a.I3-diaminopropionic acid formed was determined using the specific enzyme diaminopropionate-ammonia lyase. As little as 50 nm (5 ~g) of L-a,l3-diaminopropionic acid can be detected by the present method. The new procedure determines all acid-labile forms of bound L-a•.B-diaminopropionic acid and extracts of Cit:er arietinum and Cajanus cajun do not interfere in the procedure.

Item Type: Article
Uncontrolled Keywords: Lathyrus sativus, neurotoxic, Kesari dhal
Subjects: 600 Technology > 01 Medical sciences > 17 Toxicology
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Sep 2013 09:37
Last Modified: 20 Sep 2013 09:37
URI: http://ir.cftri.com/id/eprint/2596

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