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Antioxidant Activities of Grape (Vitis vinifera) Pomace Extracts

Chidambara Murthy, K. N. and Ravendra, P. Singh and Jayaprakasha, G. K. (2002) Antioxidant Activities of Grape (Vitis vinifera) Pomace Extracts. Journal of Agricultural and Food Chemistry, 50 (21). pp. 5909-5914.

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Abstract

<p align="justify">Antioxidant-rich fractions were extracted from grape ( Vitis vinifera) pomace using ethyl acetate,methanol, and water. The extracts were screened for their potential as antioxidants in different models. The ethyl acetate, methanol, and water extracts showed 76, 87.1, and 21.7% antioxidant activities at 100 ppm, respectively, using the 1,1-diphenyl-2-picrylhydrazyl model system. As the methanol extract of grape pomace showed maximum antioxidant activity among all of the extracts, it was selected to determine its effect on lipid peroxidation, hydroxyl radical scavenging activity, and human low-density lipoprotein (LDL) oxidation. The methanol extract showed 71.7, 73.6, and 91.2% inhibition using the thiobarbituric acid method, hydroxyl radical scavenging activity, and LDL oxidation, respectively, at 200 ppm. Treatment of albino rats of the Wistar strain with a single dose of CCl4 at 1.25 mL/kg of body weight decreases the activities of catalase, superoxide dismutase (SOD), and peroxidase by 81, 49, and 89%, respectively, whereas the lipid peroxidation value increased nearly 3-fold.Pretreatment of the rats with the methanolic extract of grape pomace at 50 mg/kg (in terms of catechin equivalents) followed by CCl4 treatment causes restoration of catalase, SOD, and peroxidase by 43.6, 73.2, and 54%, respectively, as compared with control, whereas lipid peroxidation was restored to values comparable with the control. Histopathological studies of the liver of different groups also support the protective effects exhibited by the methanol extract of grape pomace by restoring the normal hepatic architecture. Owing to this property, the studies on grape pomace can be further extended to exploit its possible application for the preservation of food products as well as a health supplement and neutraceutical.</p>

Item Type: Article
Uncontrolled Keywords: Vitis vinifera antioxidant activity DPPH LDL free radicals
Subjects: 600 Technology > 08 Food technology > 24 Fruits
600 Technology > 08 Food technology > 24 Fruits > 05 Grapes
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Human Resource Development
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Dec 2005
Last Modified: 30 Jun 2015 12:30
URI: http://ir.cftri.com/id/eprint/250

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