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A central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level.

Kiran, K. R. and Manohar, B. and Divakar, S. (2001) A central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level. Enzyme and Microbial Technology, 29 (2/3). 122-128, 13 ref..

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Abstract

Porcine Pancreas lipase (PPL) was employed for the reaction between lactic acid and lauric acid at bench-scale level. A Response Surface Methodological (RSM) study was conducted by employing a five-level, five variable, central composite rotatable design (CCRD) in order to understand the esterification behavior of PPL in the lauroyl lactic acid synthesis. Five important variables were considered, namely, enzyme/substrate (E/S) ratio (0.09 –1.14 Activity Units/mmole - AU/mmol), lactic acid concentration (5–25 mmol), incubation period (6–54 h), buffer volume (0–0.2 ml) and buffer pH Values (4.0–8.0). Highest ester yield of 6.8 mmol was predicted at the lowest E/S ratio of 0.09 AU/mmol. Lower E/S ratios gave higher yields and higher E/S ratios gave lesser yields. This behavior clearly explained the competitive nature of binding between lauric and lactic acids for the same binding site on the enzyme. Addition of buffer in terms of both volume and pH did not have a profound effect on increase in ester yield. Predicted yields showed good validation with experimental yields when experiments corresponding to selected points on the contour plots were carried out.

Item Type: Article
Uncontrolled Keywords: Lauroyl lactic acid; Central composite rotatable design (CCRD); Esterification reaction; Bench-scale level; Iso-esterification regions
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions: Fermentation Technology and Bioengineering
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Nov 2008 11:26
Last Modified: 28 Dec 2011 09:40
URI: http://ir.cftri.com/id/eprint/2431

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