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Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation.

Bijesh, Puthusseri and Divya, P. and Veeresh, Lokesh and Gyanendra, Kumar (2018) Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation. Journal of Agricutural and Food Chemistry, 66. pp. 505-511.

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Abstract

Increasing the quantity of natural folates in plant foods is recently gaining significant interest, owing to their acute deficiencies in various populations. This study observed that foliar salicylic acid treatment enhanced the accumulation of folates in Arabidopsis, which correlated with the increase in a folate binding protein (FBP) and the expression of mRNA of a putative folate binding protein At5G27830. A protein band corresponding to ∼43 kDa was observed after resolving the affinitypurified protein on SDS-PAGE, and the partial amino acid sequence indicated that the protein is indeed At5G27830. Docking studies performed with At5G27830 confirmed specific binding of folic acid to predicted site. Heterologous expression of At5G27830 in the yeast resulted in significant uptake and accumulation of folic acid in cells. This novel study of a plant FBP will be useful for folate metabolic engineering of a wide range of crops.

Item Type: Article
Uncontrolled Keywords: folate binding protein, folate receptor, At5G27830, plant folate, metabolic engineering
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions: Dept. of Biochemistry
Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Feb 2018 04:02
Last Modified: 20 Feb 2018 04:02
URI: http://ir.cftri.com/id/eprint/2384

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