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Fuel smoke condensate induced DNA damage in human lymphocytes and protection by turmeric (Curcuma longa).

Shalini, V. K. and Srinivas, L. (1990) Fuel smoke condensate induced DNA damage in human lymphocytes and protection by turmeric (Curcuma longa). Molecular and Cellular Biochemistry, 95 (1). pp. 21-30. ISSN 0300-8177

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Abstract

Twigs-dry leaves smoke condensate (TDS) was investigated for its DNA damaging activity in human peripheral lymphocytes, by using a sensitive method, fluorescence analysis of DNA unwinding (FADU). An aqueous turmeric component (Aq.T) was studied as a protective agent. TDS at one to 100 folds dilution induced 55% DNA damage at 20 min, while 12-O-tetradecanoylphorbol-13-acetate (TPA) at 10 ng/ml induced only 25% damage. Aq.T at 300 ng/microliter afforded 90% protection to DNA against TDS and 65% against TPA. The mechanism of Aq.T protection was investigated by using (i) inhibitors of arachidonate cascade, viz., indomethacin (28 microM), NDGA (10 microM), DBAP (36 microM), (ii) antioxidant enzymes viz., CAT (0.2 U/microliter), SOD (0.6 U/microliter), (iii) antioxidants--BHA, curcumin (40 microM), mixed gangliosides (20 nM) and protease inhibitor TLCK (100 microM). These compounds offered the following extents of protection to DNA against TDS: indomethacin--40%, NDGA--83%, DBAP--70%, SOD--38%, CAT--40%, BHA--38%, curcumin--60%, mixed gangliosides--88%, TLCK--85%. Against TPA as clastogenic agent, the extents of protection were: indomethacin--73%, NDGA--32%, DBAP--72%, SOD--60%, CAT, BHA-negligible, curcumin--23%, mixed gangliosides--60%, TLCK--59%. These results indicate that (i) TDS and TPA induce DNA damage possibly by different mechanisms, (ii) Aq.T is a more effective protectant against TDS whereas it is on par with other inhibitors against TPA.

Item Type: Article
Uncontrolled Keywords: fuel smoke, DNA damage, FADU, turmeric protection
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
600 Technology > 08 Food technology > 30 Spices/Condiments > 07 Turmeric
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Mar 2018 08:55
Last Modified: 20 Mar 2018 08:55
URI: http://ir.cftri.com/id/eprint/2345

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