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Assessment of in vivo mutagenic potency of ethylenediaminetetraacetic acid in albino mice.

Dr., Muralidhara and Narasimhamurthy, K. (1991) Assessment of in vivo mutagenic potency of ethylenediaminetetraacetic acid in albino mice. Food and Chemical Toxicology , 29 (12). pp. 845-9. ISSN 0278-6915

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Abstract

Ethylenediaminetetraacetic acid (EDTA) disodium salt, a widely used metal chelator, was studied for its potency to induce bone marrow micronuclei, dominant lethal mutations and sperm-head abnormalities in albino mice. The acute oral LD50 dose computed by probit regression was 30 mg/kg body weight in the strain used. Preliminary studies showed that oral administration of EDTA disodium salt at doses of 5, 10 and 15 mg/kg body weight/day on 5 consecutive days did not induce any obvious signs of toxicity. In the bone marrow micronucleus assay acute doses of EDTA disodium salt (5-20 mg/kg body weight) induced a dose-dependent increase in the incidence of micronucleated polychromatic erythrocytes at a 24-hr sampling. However, administration at doses of 5, 10 and 15 mg/kg for 5 consecutive days did not produce any observable effect on either the testicular or epididymal weights and histology. No appreciable alterations were observed in the caudal sperm counts at any of the sampling intervals and there was no treatment-related increase in the incidence of sperm-head abnormalities. Furthermore, treatment of male mice with EDTA disodium salt (10 mg/kg body weight/day for 5 consecutive days) induced no increase in the incidence of post-implantation embryonic deaths, except for a marginal but statistically insignificant increase during wk 2 and 3 of mating.

Item Type: Article
Uncontrolled Keywords: Ethylenediaminetetraacetic acid, metal chelator, genetic toxicology
Subjects: 600 Technology > 08 Food technology > 31 Food Additives
600 Technology > 08 Food technology > 12 Allergy & Toxicology
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 09 Jun 2011 08:55
Last Modified: 28 Dec 2011 09:40
URI: http://ir.cftri.com/id/eprint/2322

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