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Detergent permeabilized yeast cells as the source of intracellular enzymes for estimation of biomolecules.

Bhat, N. and Naina, N. S. and Gowda, L. R. and Bhat, S. G. (1993) Detergent permeabilized yeast cells as the source of intracellular enzymes for estimation of biomolecules. Enzyme and Microbial Technology, 15 (9). pp. 796-800. ISSN 0141-0229

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Abstract

The feasibility of using permeabilized whole cells as a source of intracellular enzymes instead of isolated expensive enzymes for the estimation of biomolecules has been studied. Alcohol dehydrogenase (ADH), glucose-6-phosphate dehydrogenase (G6PDH), hexokinase (HK), and beta-galactosidase (beta-GAL) activities of cetyltrimethylammonium bromide (CTAB)-permeabilized whole yeast cells were employed to estimate ethyl alcohol, glucose, and lactose. The method using permeabilized cells was comparable to that of isolated enzymes and was applicable for the estimation of these analytes in complex samples such as blood, milk, and fermented samples. The usefulness of permeabilized cells as a single source of more than one enzyme required for coupled enzyme assays was demonstrated.

Item Type: Article
Uncontrolled Keywords: Yeast; detergent permeabilization; intracellular enzymes; analytical reagent
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast
600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Mar 2011 09:22
Last Modified: 18 Nov 2016 10:49
URI: http://ir.cftri.com/id/eprint/2284

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