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A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae.

Shankar, C. S. and Umesh Kumar, S. (1994) A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae. Microbiology, 140. pp. 1097-101. ISSN 1350-0872

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Abstract

A cell-wall-surface protein purified from the cells of Saccharomyces cerevisiae NCYC 227 was found to be involved in the non-sexual flocculation of this yeast. This 13 kDa protein was found to bind specifically to mannose. The protein bound to mannans isolated from yeast as well as in situ to intact cells, but only in the presence of calcium ions. The protein, a mannoprotein, formed aggregates as revealed in SDS-PAGE. Urea and higher temperatures prevented protein aggregation, suggesting that the flocculation of S. cerevisiae is primarily due to hydrogen bonding between mannan and protein.

Item Type: Article
Uncontrolled Keywords: Saccharomyes cerevisiae, flocculation, lectin
Subjects: 600 Technology > 08 Food technology > 29 Microbiological food > 04 Yeast
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Mar 2018 08:37
Last Modified: 21 Mar 2018 08:37
URI: http://ir.cftri.com/id/eprint/2275

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