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Isolation and characterization of a sialo-glycopeptide from buffalo colostrum.

Aparna, H. S. and Salimath, P. V. (1996) Isolation and characterization of a sialo-glycopeptide from buffalo colostrum. Glycoconjugate Journal, 13 (1). pp. 63-7. ISSN 0282-0080

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Abstract

A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose, N-acetyl glucosamine and N-acetyl neuraminic acid in the ratio 1:2:3:4:1, and aspartic acid, serine, threonine, proline and glutamic acid were the major amino acids. Glycine was identified as the N-terminal amino acid residue. The structure elucidation of the carbohydrate moiety was carried out by methylation analysis, mass spectrometry. 1H-NMR spectroscopy and the probable structure was revealed to be that of a complex biantennary type.

Item Type: Article
Uncontrolled Keywords: buffalo, colostrum, glycopeptide
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry
600 Technology > 03 Agriculture > 03 Animal Husbandry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Mar 2018 05:16
Last Modified: 22 Mar 2018 05:16
URI: http://ir.cftri.com/id/eprint/2241

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