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Development of modified plant tissue culture media using alternate sources of nitrogen and vitamins for micropropagation.

George, J. and Ravishankar, G. A. (1996) Development of modified plant tissue culture media using alternate sources of nitrogen and vitamins for micropropagation. Indian Journal of Experimental Biology, 34. pp. 163-170.

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Abstract

Vegetative bud explants from Musa paradisiaca William cv. silk, Gardenia jasminoides Ellis and Menthc piperita were cultured on nutrient media using an alternate nitrogen source which is a composite fertilizer "Suphala". A cyanobacterium, Spirulina platensis which is a source of vitamins, was also incorporated at various levels and the effects of these were studied by monitoring the shoot number, shoot length and number of leaves over a period of 30 days. Medium M2 produced a maximum of 5 shoots per explant in case of Gardenia whereas SM2 produced the similar number in case of Musa. Spirulina incorporated in half-strength MS media produced an average of 3 shoots each in Musa and Mentha. The results show that incorporation of these alternative nitrogen and vitamin sources in the media could be effective in cost minimization for micropropagation of these plants.

Item Type: Article
Uncontrolled Keywords: Musa paradisiaca, Gardenia jasminoides, Mentha piperita, micropropagation, plant tissue culture
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Apr 2012 09:03
Last Modified: 02 Aug 2016 10:38
URI: http://ir.cftri.com/id/eprint/2234

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