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Studies on the inactivation of superoxide dismutase activity by nitric oxide from rat peritoneal macrophages.

Joe, B and Lokesh, B. R. (1997) Studies on the inactivation of superoxide dismutase activity by nitric oxide from rat peritoneal macrophages. Molecular and Cellular Biochemistry, 168 (1-2). pp. 87-93. ISSN 0300-8177

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Abstract

Rat peritoneal macrophages stimulated with lipopolysaccharide (LPS) and Phorbol myristate acetate (PMA) generated increased levels of superoxide anions (O2.-) by 122% as compared to those stimulated with PMA alone. However, Nitric oxide (NO) synthase inhibitors-n-monomethyl arginine (nMMA) or spermine-HCI lowered the enhanced levels of O2.- released by LPS treated macrophages. The Superoxide dismutase (SOD) activity in LPS treated macrophages was 51% lower than that observed in resident cells. NO synthase inhibitors prevented the loss of SOD activity in LPS treated cells. Exogenously added SOD during sensitization of cells with LPS also inactivated the enzyme. This inactivation of SOD is inhibited by Nitric oxide synthase inhibitors. PMA alone did not affect SOD activity. NO synthase inhibitors also did not affect PMA activated superoxide anion generation in macrophages. These studies indicate that nitric oxide generated by LPS treated macrophages can inactivate SOD activity.

Item Type: Article
Uncontrolled Keywords: nitric oxide, superoxide dismutase, macrophages
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 01 Jul 2009 11:37
Last Modified: 28 Dec 2011 09:39
URI: http://ir.cftri.com/id/eprint/2224

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