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Acoustic demixing of aqueous two-phase systems

Srinivas, N. D. and Barhate, R. S. and Raghavarao, K. S. M. S. and Todd, P. (2000) Acoustic demixing of aqueous two-phase systems. Applied Microbiology and Biotechnology, 53. pp. 650-654.

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<p align="justify">Aqueous two-phase systems demix slowly due to similar physical properties. This is one of the major drawbacks for their adaptation for industrial scale extraction of enzymes. In the present work, a method to accelerate the demixing rates of these systems, employ- ing a traveling acoustic wave ®eld is reported for the ®rst time. Phase-demixing for three systems, viz. polyethyl- ene glycol (PEG)/sodium sulfate, PEG/potassium phosphate and PEG/maltodextrin were studied. The acoustically assisted process decreased the demixing time signi®cantly (about 2- to 3-fold in PEG/salt systems and about 2-fold in the PEG/maltodextrin system), com- pared to that in gravity alone. Ultrasonication appar- ently enhanced the coalescence of the dispersed phase droplets due to the mild circulation currents it caused in the dispersion. This in turn enhanced the rate of demixing due to the increased migration velocity of the larger droplets.</p>

Item Type: Article
Uncontrolled Keywords: demixing aqueous two phase systems
Subjects: 600 Technology > 08 Food technology > 07 Food Engineering
600 Technology > 08 Food technology > 14 Physical properties
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Nov 2005
Last Modified: 28 Dec 2011 09:25
URI: http://ir.cftri.com/id/eprint/219

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