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Genetic improvement of Aspergillus carbonarius for pectinase overproduction during solid state growth.

Kavitha, R. and Umesh Kumar, S. (2000) Genetic improvement of Aspergillus carbonarius for pectinase overproduction during solid state growth. Biotechnology and bioengineering, 67 (1). pp. 121-5. ISSN 0006-3592

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Abstract

Low pectinase production by Aspergillus carbonarius growing on wheat bran solid substrate was found to be due to reduced colonizational ability of the fungus. Since A. niger showed higher growth rates on wheat bran, strain improvement to obtain higher pectinase production in solid state was carried out by inter-specific fusion of protoplasts of A. carbonarius and A. niger. One of the mutants selected for higher activities of alpha-glucosidase showed improved growth rates on wheat bran solid substrate together with increased pectinase production. Size similarities of amplified polymorphic DNA of the mutant with the two parents and identification of a 66 kDa polygalacturonase specific to A. niger suggested genetic recombination in the mutant.

Item Type: Article
Uncontrolled Keywords: Aspergillus carbonarius;pectinase; strain improvement; protoplant fusion; solid-state fermentation
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Aug 2008 10:53
Last Modified: 01 Oct 2018 10:54
URI: http://ir.cftri.com/id/eprint/2182

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