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Structural studies of a glucan isolated from blue-green alga Spirulina platensis

Madhavi Sekharam, K. and Venkataraman, L. V. and Salimath, P. V. (1989) Structural studies of a glucan isolated from blue-green alga Spirulina platensis. Food Chemistry, 31 (2). pp. 85-91. ISSN 0308-8146

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Abstract

It has been suggested that blue-green alga could provide a source of unconventional protein for use in foods and feeds. This study examined the structure and chemical nature of a glucan isolated from this alga. The alga was cultivated in fresh water (with bicarbonate as C source and NaNO3 as N source), harvested by filtration, washed, freeze-dried, defatted and sequentially extracted with 70% alcohol and cold and hot water. The insoluble residue was extracted with HCl at 70 degree C, the extracts being pooled, dialysed, concentrated, precipitated with alcohol and dried. The acid-soluble polysaccharide obtained was subjected to methylation analysis, periodate oxidation, CrO3 oxidation and enzymatic analysis. The results obtained indicated that the acid-soluble polysaccharide consists principally of glucose with small amounts of sulphate. It was found to have an alpha-1,4-linked glucan backbone with frequent side-chains through alpha-1,6-linked glucosyl residues.

Item Type: Article
Uncontrolled Keywords: ALGAE-; Spirulina platensis glucans, structure of; POLYSACCHARIDES-
Subjects: 600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jul 2008 04:14
Last Modified: 21 Dec 2016 12:54
URI: http://ir.cftri.com/id/eprint/2163

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