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Unfolding of multimeric proteins in presence of denaturants. A case study of helianthinin from Helianthus annuus L.

Suryaprakash, P. and Prakash, V. (2000) Unfolding of multimeric proteins in presence of denaturants. A case study of helianthinin from Helianthus annuus L. Die Nahrung, 44 (3). pp. 178-83. ISSN 0027-769X

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Abstract

Helianthinin (11S), a multisubunit protein from Sunflower Seeds (Helianthus annuus L.) dissociates to its monomer (2S) through a trimeric (7S) intermediate as a function of guanidine hydrochloride (GuHCl) and guanidine thiocyanate (GuHSCN) concentration. Measurements of viscosity, velocity sedimentation patterns and spectroscopic parameters of the protein in presence of these denaturants both at equilibrium and as a function of time clearly suggest that the dissociation, unfolding and aggregation of this multimeric protein occur sequentially. The unfolding of the protein in guanidinium salts has two transitions with the first transition occurring between native to intermediate and the second transition occurring between intermediate state and unfolded state. The midpoint concentration for the major transition is 1.75 M for GuHCl and 0.8 M for GuHSCN. Evaluation of this data suggests that during the process of denaturation the simultaneous unfolding of acidic and basic subunits of the protein takes place. At intermediate concentrations of denaturant namely 1.6 M of GuHCl or 1.1 M of GuHSCN the aggregation of the protein was found to be maximum. The results suggests the possibility of a mechanism for the dissociation, denaturation and unfolding of multimeric proteins in presence of the chemical denaturants.

Item Type: Article
Uncontrolled Keywords: Sunflower Seeds, Helianthus annuus L.), Helianthinin, protein
Subjects: 600 Technology > 08 Food technology > 19 Lipids-oils/fats > Sunflower Seed
600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Jul 2011 04:17
Last Modified: 28 Dec 2011 09:39
URI: http://ir.cftri.com/id/eprint/2157

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