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Amyloglucosidase-catalyzed synthesis of n-octyl-d-glucoside— analysis using response surface methodology

Giriyapura, R. Vijayakumar and Manohar, B. and Divakar, S. (2004) Amyloglucosidase-catalyzed synthesis of n-octyl-d-glucoside— analysis using response surface methodology. European Food Research and Technology, 220. pp. 272-277.

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Abstract

<p align="justify">Amyloglucosidase (3.2.1.3)-catalyzed synthesis of n-octyl-d-glucoside was optimized using response surface methodology. A central composite rotatable design involving 32 experiments of five variables at five levels was employed to study the glucosylation reaction. Among the variables studied, namely, n-octanol (15– 75 MEq to d-glucose), enzyme (20–100 mg), pH (4.0– 8.0), buffer volume (0.2–1.0 ml) and temperature (30– 70_C), amyloglucosidase concentration, pH and temperature were found to be significant. Experimental data fitted the second-order polynomial equation well, as indicated by an R2 value of 0.895. Validation experiments carried out under predicted conditions showed good correspondence between experimental and predicted yields. Various surface plots were generated to describe the relationship between operating variables and the conversion yields. The highest yield of 53.5% predicted at optimum conditions of 75 Eq n-octanol, 20 mg amyloglucosidase, 0.2 ml, pH 7.8 buffer at 50 _C showed good correspondence to the experimental yield of 53.8% under these conditions.</p>

Item Type: Article
Uncontrolled Keywords: n-Octyl-d-glucoside Amyloglucosidase n-Octanol pH Buffer volume
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Nov 2005
Last Modified: 07 May 2012 05:03
URI: http://ir.cftri.com/id/eprint/214

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