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Drop formation in aqueous two-phase systems.

Barhate, R. S. and Patil, Ganapathi and Srinivas, N. D. and Raghavarao, K. S. M. S. (2004) Drop formation in aqueous two-phase systems. Journal of Chromatography. A, 1023 (2). pp. 197-206. ISSN 0021-9673

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Abstract

Extraction using aqueous two-phase systems (ATPSs) is a versatile technique for the downstream processing of various proteins/enzymes. The study of drop formation deals with the fundamental understanding of the behavior of liquid drops under the influence of various external body as well as surface forces. These studies provide a basis for designing of the extractions in column contactors in which liquid drops play a major role. Most of the drop formation studies reported so far is restricted to aqueous-organic systems. ATPSs, differ from aqueous-organic systems in their physical properties. In view of this, an attempt was made to develop a model for drop formation in ATPSs adopting the information available on aqueous-organic systems. In order to validate the model, experiments were performed by using polyethylene glycol (PEG)/salt systems of different phase compositions at various flow rates. At low flow rates the single stage model and at high flow rates the two stage model are able to predict the drop volume during its formation from tip of capillary. The experimental results were found to agree reasonably well with those predicted by the model.

Item Type: Article
Uncontrolled Keywords: Aqueous two-phase system; Drop formation
Subjects: 600 Technology > 08 Food technology > 07 Food Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Aug 2008 09:39
Last Modified: 01 Jul 2015 12:42
URI: http://ir.cftri.com/id/eprint/2043

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