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Production of thiophenes from callus cultures of Tagetes patula L. and its mosquito larvicidal activity.

Rajasekaran, T. and Ravishankar, G. A. and Obul Reddy, B. (2003) Production of thiophenes from callus cultures of Tagetes patula L. and its mosquito larvicidal activity. Indian Journal of Experimental Biology, 41 (1). pp. 63-68. ISSN 0019-5189

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Abstract

Calli were initiated from leaf tissues of T. patula, on Murashige and Skoog's medium supplemented with 2-4 dichlorophenoxacetic acid (2,4-D) and kinetin (Kn). The maximum accumulation of biomass was recorded on 6th week at 1.11 g. dry wt/culture. Maximum thiophene content was recorded on 4th week at 0.008% on dry wt basis in callus cultures of T. patula subjected to nutrient stress. Nitrogen stress induced 3-fold increase in thiophene production level in six weeks (0.024% on dry weight basis). The best hormonal supplementation required for thiophenes production was found to be 2,4-D (2.0 mg L(-1)) and kinetin (2.0 mg L(-1)). The thiophenes produced in callus cultures of T. patula showed larvicidal effect against mosquito larvae.

Item Type: Article
Uncontrolled Keywords: Marigold; GC analysis; GC-MS
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Users 197 not found.
Date Deposited: 08 Jul 2011 09:05
Last Modified: 08 Jul 2011 09:05
URI: http://ir.cftri.com/id/eprint/2028

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