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Chitooligosaccharides--preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity.

Kittur, Farooqahamed S and Vishu Kumar, Acharya B and Varadaraj, M. C. and Tharanathan, R. N. (2005) Chitooligosaccharides--preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity. Carbohydrate research, 340 (6). pp. 1239-45. ISSN 0008-6215

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Abstract

An isozyme of pectinase from Aspergillus niger with polygalacturonase activity caused chitosanolysis at pH 3.5, resulting in low-molecular weight chitosan (86%), chitooligosaccharides (COs, 4.8%) and monomers (2.2%). HPLC showed the presence of COs with DP ranging from 2 to 6. Charcoal-Celite chromatography and re-N-acetylation of the COs followed by CD, IR, MALDI-TOF-MS and FAB-MS analyses revealed an abundance of chitobiose, chitotriose and chitotetraose. The COs-monomeric mixture showed a bactericidal effect towards Bacillus cereus and Escherichia coli more efficiently than native chitosan. Among the chitooligomers, the hexamer showed maximum antibacterial effect followed by the penta-, tetra-, tri- and dimers. Of the two monomers, only GlcN showed slight bacterial growth inhibition. SEM revealed bactericidal action patterns of COs-monomeric mixture towards B. cereus and E. coli.

Item Type: Article
Uncontrolled Keywords: Chitosan; Pectinase; Chitooligosaccharides; MALDI-TOF-MS; FAB-MS; Antibacterial activity
Subjects: 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions: Dept. of Biochemistry
Human Resource Development
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Nov 2008 09:10
Last Modified: 16 May 2012 06:49
URI: http://ir.cftri.com/id/eprint/1996

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