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Synthesis of guaiacol-alpha-D-glucoside and curcumin-bis-alpha-D-glucoside by an amyloglucosidase from Rhizopus

Vijayakumar, G. R. and Divakar, S. (2005) Synthesis of guaiacol-alpha-D-glucoside and curcumin-bis-alpha-D-glucoside by an amyloglucosidase from Rhizopus. Biotechnology Letters, 27. pp. 1411-1415. ISSN 0141-5492

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Abstract

Guaiacol (2-methoxyphenol) and curcumin [1E,6E-1,7-di(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione] were converted into their corresponding glucosides using glucose and an amyloglucosidase from Rhizopus. Guaiacol-alpha-D-glucoside yields ranged from 3 to 52% with the highest at pH 7.0. Curcumin-bis-alpha-D-glucoside yields ranged from 3 to 48% with the highest at pH 4.0 with 50% (w/w D-glucose) of enzyme. The phenolic hydroxyl group of guaiacol and both phenolic hydroxyl groups of curcumin were glucosylated at the C1 carbon of alpha-D-glucose indicating that the enzymatic reaction is stereospecific. Both guaiacol-alpha-D-glucoside and curcumin-bis-alpha-D-glucosides had antioxidant activities.

Item Type: Article
Uncontrolled Keywords: 2-methoxyphenol; E,6E-1,7-di(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione; Guaiacol-alpha-D-glucoside; Glucose
Subjects: 600 Technology > 08 Food technology > 10 Food Microorganisms
600 Technology > 08 Food technology > 31 Food Additives
600 Technology > 08 Food technology > 30 Spices/Condiments > 07 Turmeric
600 Technology > 08 Food technology > 11 Food Biochemistry
600 Technology > 08 Food technology > 30 Spices/Condiments
600 Technology > 08 Food technology > 08 Food Chemistry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Users 197 not found.
Date Deposited: 01 Jun 2011 05:55
Last Modified: 01 Jun 2011 05:55
URI: http://ir.cftri.com/id/eprint/1970

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