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Response surface methodological study of esterification of lactic acid with palmitic acid catalyzed by immobilized lipases from Mucor miehei and porcine pancreas

Kondabagil, R. Kiran and Balaraman, Manohar and Nayakanakatte, G. Karanth and Soundar, Divakar (2000) Response surface methodological study of esterification of lactic acid with palmitic acid catalyzed by immobilized lipases from Mucor miehei and porcine pancreas. European Food Research and Technology, 211. pp. 130-135.

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Abstract

<p align="justify">Response surface methodology was employed to analyze the effects of important process parameters such as lactic acid/palmitic acid (LA/PA) concentrations (Xs), enzyme/substrate (E/S) ratios (Xe) and incubation time (Xt) on the preparation of palmitoyl lactic acid using Mucor miehei lipase (MML) and porcine pancreas lipase (PPL). Experimental ester yields were found to be in good agreement with predictions. In the case of MML, the ester yield was found to increase with Xe and Xt and a maximum yield of 27 mM was obtained at Xe = 128 activity units(AU)/mM of substrate, Xs = 64±74 mM and Xt = 72 h. In the case of PPL, the ester yield was found to increase with increases in Xe and Xs. Optimum conditions for PPL were Xe = 45 AU/mM,Xs = 85±90 mM and Xt = 72 h, at which the maximum ester yield of 15 mM was obtained.</p>

Item Type: Article
Uncontrolled Keywords: Palmitoyl lactic acid Enzyme/substrateratios ¥ Lactic acid/palmitic acid concentrations Iso-esterification regions
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Sep 2005
Last Modified: 28 Dec 2011 09:25
URI: http://ir.cftri.com/id/eprint/197

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