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Influence of 8-methyl-nonenoic acid on capsaicin biosynthesis in in vivo and in vitro cell cultures of Capsicum spp.

Narasimha Prasad, B. C. and Gururaj, Harishchandra Bhaskar and Vinod, Kumar and Giridhar, Parvatam and Parimalan, Rangan and Sharma, Ashwani and Ravishankar, G. A. (2006) Influence of 8-methyl-nonenoic acid on capsaicin biosynthesis in in vivo and in vitro cell cultures of Capsicum spp. Journal of Agricultural and Food Chemistry, 54. pp. 1854-1859. ISSN 0021-8561

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Abstract

Capsaicin is a bioactive molecule synthesized by enzymatic (putative capsaicin synthase) condensation of vanillylamine, a phenyl propanoid intermediate with 8-methyl-nonenoic acid, a fatty acid derivative from leucine/valine pathway. Analysis of levels of 8-methyl-nonenoic acid and phenyl propanoid intermediates in high, medium, and low pungent Capsicum genotypes revealed that the 8-methyl-nonenoic acid pool plays a crucial role in determining the efficacy of capsaicin levels. Cerulenin-mediated inhibition of 8-methyl-nonenoic acid synthesis decreased the capsaicin biosynthesis in Capsicum cell suspension cultures. Similarly amino oxy acetate inhibited vanillylamine synthesis but failed to reduce capsaicin production. The mRNA transcript analysis of keto acyl synthase (KAS), a crucial enzyme involved in 8-methyl-nonenoic acid and an amino transferase (AMT), involved in vanillylamine biosynthesis was studied. The mRNA transcript analysis revealed the progressive developmental expression of KAS gene in the placenta during the ontogeny of the fruit, whereas AMT transcripts levels did not show significant differences. Hence, the study demonstrates the influence of 8-methyl-nonenoic acid and its possible regulatory role in capsaicin biosynthesis in Capsicum spp.

Item Type: Article
Uncontrolled Keywords: capsaicin synthase; vanillylamine; 8-methyl-nonenoic acid; mRNA transcripts; cerulenin; amino oxyacetate
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
500 Natural Sciences and Mathematics > 10 Plants > 01 Plant Cell
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
600 Technology > 08 Food technology > 08 Food Chemistry
Divisions: Plant Cell Biotechnology
Depositing User: Users 16 not found.
Date Deposited: 01 Jun 2011 06:04
Last Modified: 05 Oct 2018 05:55
URI: http://ir.cftri.com/id/eprint/1941

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