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Studies on genomic DNA topology and stability in brain regions of Parkinson's disease.

Muralidhar Hegde, L. and Gupta, V. B. and Anitha, M. and Harikrishna, T. and Shankar, S. K. and Muthane, U. and Subba Rao, K. and Jagannatha Rao, K. S. (2006) Studies on genomic DNA topology and stability in brain regions of Parkinson's disease. Archives of Biochemistry and Biophysics, 449 (1-2). pp. 143-156. ISSN 0003-9861

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Abstract

DNA damage has been postulated as a mechanism of neuronal death in Parkinson's disease (PD). In the present study, genomic DNA was isolated from eight brain regions (frontal, temporal, and occipital cortex, hippocampus, caudate/putamen, thalamus, cerebellum, and midbrain) from five neuropathologically confirmed cases of Parkinson's disease and six control brains and analyzed for the presence of single and double strand breaks, melting temperature, EtBr intercalation, DNAse digestion pattern, and DNA conformations. The results showed that DNA from midbrain in PD accumulated significantly higher number of strand breaks than age-matched controls. Caudate nucleus/putamen, thalamus, and hippocampus also showed more DNA fragmentation compared to control brains. Circular dichroism studies showed that DNA conformation was altered with imprecise base stacking in midbrain, caudate nucleus/putamen, thalamus, and hippocampus in PD. However, DNA from frontal, temporal, and occipital cortex, and cerebellum was not affected significantly in PD group as compared to controls. This study provides a comprehensive database on stability, damage, and conformations of DNA in different regions in brains of PD patients.

Item Type: Article
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
500 Natural Sciences and Mathematics > 07 Life Sciences > 02 Evolution & genetics
Divisions: Dept. of Biochemistry
Depositing User: Users 16 not found.
Date Deposited: 19 Jul 2011 06:22
Last Modified: 19 Jul 2011 06:22
URI: http://ir.cftri.com/id/eprint/1937

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