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Method to obtain C-phycocyanin of high purity.

Patil, Ganapathi and Chethana, S. and Sridevi, A. S. and Raghavarao, K. S. M. S. (2006) Method to obtain C-phycocyanin of high purity. Journal of chromatography. A, 1127 (1-2). pp. 76-81. ISSN 0021-9673

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Abstract

A new approach is made for the purification of C-phycocyanin (C-PC), which is simple and more efficient than existing methods. The proposed method involves two steps: aqueous two phase extraction and ion-exchange chromatography. Crude extract of C-phycocyanin, of purity 1.18, obtained from Spirulina platensis is subjected to aqueous two phase extraction. C-phycocyanin obtained from this process showed a purity of 5.22, which is higher than the reported value till date. In order to explore the possibility of further purification, C-phycocyanin is subjected to ion-exchange chromatography and found that the purity increased from 5.22 to 6.69. The fluorescence, intactness of structure and purity of C-phycocyanin are confirmed using spectrofluorometry, circular dichroism spectra and sodium dodecyl sulfate-polyacrylamide gel, respectively.

Item Type: Article
Uncontrolled Keywords: C-phycocyanin; Aqueous two phase extraction; Adsorption; Purification; Ion-exchange chromatography
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Food Engineering
Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 05 Aug 2008 11:37
Last Modified: 04 Sep 2018 11:36
URI: http://ir.cftri.com/id/eprint/1932

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