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Culture media optimization for growth and phycoerythrin production from Porphyridium purpureum.

Kathiresan, S. and Sarada, R. and Sila, Bhattacharya and Ravishankar, G. A. (2007) Culture media optimization for growth and phycoerythrin production from Porphyridium purpureum. Biotechnology and bioengineering, 96 (3). pp. 456-63. ISSN 0006-3592

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Abstract

Porphyridium spp. is a red micro alga and is gaining importance as a source of valuable products viz., phycobiliproteins (PB), sulfated exopolysaccharides, and polyunsaturated fatty acids with potential applications in the food and pharmaceutical industries. In the present study, the effects of the major media constituents of Porphyridium species were studied using response surface methodology (RSM) on biomass yield, total PB and the production of phycoerythrin (PE). A second order polynomial can be used to predict the PB and PE production in terms of the independent variables. The independent variables such as the concentrations of sodium chloride, magnesium sulfate, sodium nitrate, and dipotassium hydrogen phosphate influenced the total PB and PE production. The optimum conditions showed that total PB was 4.8% at the concentration of sodium chloride 26.1 g/L, magnesium sulfate 5.23 g/L, sodium nitrate 1.56 g/L, and dipotassium hydrogen phosphate 0.034 g/L. In case of optimum PE production (3.3%), the corresponding values are 29.62, 6.11, 1.59, and 0.076 g/L, respectively. PE production depends greatly on the concentrations of chloride, nitrate, and sulfate as well as phosphate of which the former possess the maximum effect.

Item Type: Article
Uncontrolled Keywords: Porphyridium, Phycobiliproteins (PB), Phycoerythrin (PE), Biomass,Response surface methodology (RSM), Optimization
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Grain Science and Technology
Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Aug 2008 06:11
Last Modified: 01 Oct 2018 10:47
URI: http://ir.cftri.com/id/eprint/1912

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