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Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties.

Dhale, M. A. and Divakar, S. and Umesh Kumar, S. and Vijayalakshmi, G. (2007) Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties. Applied Microbiology and Biotechnology, 73 (5). pp. 1197-202. ISSN 0175-7598

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Abstract

The methanolic extract of Monascus purpureus cultivated by solid-state fermentation on rice showed strong 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and better yield as compared to other polarity based extracted fractions. It was selected for further purification of the antioxidant. The activity-guided repeated fractionation of methanolic extract on a silica gel column chromatography yielded a compound that exhibited strong antioxidant activity. Based on the spectroscopic analysis by UV, IR, 1H NMR, 13C NMR, 2D-HSQCT NMR, and MS, the antioxidant isolated was elucidated as a derivative of dihydromonacolin-K, where the ester group is 2-methyl propionate, designated as dihydromonacolin-MV. The DPPH radical was significantly scavenged by the dihydromonacolin-MV (IC50 20+/-1 microg ml-1). The dihydromonacolin-MV showed strong inhibition of lipid peroxidation in a liposome model with an IC50 value of 5.71+/-0.38 microg ml-1 and superoxide radical scavenging activity with an IC50 value of 163.97+/-2.68 microg ml-1.

Item Type: Article
Uncontrolled Keywords: Monascus purpureus Dihydromonacolin-MV Antioxidant DPPH lipid peroxidation Superoxide radical
Subjects: 600 Technology > 08 Food technology > 21 Cereals > 01 Rice
600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Fermentation Technology and Bioengineering
Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Sep 2010 09:23
Last Modified: 11 May 2012 04:22
URI: http://ir.cftri.com/id/eprint/1907

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