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Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage.

Harish Prashanth, K. V. and Shylaja, M. Dharmesh and Jagannatha Rao, K. S. and Tharanathan, R. N. (2007) Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage. Carbohydrate research, 342 (2). pp. 190-5. ISSN 0008-6215

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Abstract

Low molecular weight chitosan (LMWC) and chitooligosaccharides (COs), obtained by persulfate-induced depolymerization of chitosan showed scavenging of OH. and O2.- radicals and offered protection against calf thymus DNA damage. Over 85% inhibition of free radicals and DNA protection were observed. LMWC (0.05 micromol) showed a strong inhibitory activity compared to COs (3.6 micromol). Further, LMWC showed calf thymus DNA condensation reversibly giving stability, as evident from CD, TEM and melting curves (Tm). A fluorescence study suggests the binding of LMWC in the minor groove, forming H-bonds to the backbone phosphates without distorting the double helix structure.

Item Type: Article
Uncontrolled Keywords: Chitosan; Free radicals; Degradation products; Antioxidants; DNA condensation; Gene therapy; Transfection
Subjects: 600 Technology > 08 Food technology > 11 Food Biochemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Aug 2008 11:54
Last Modified: 30 May 2012 07:05
URI: http://ir.cftri.com/id/eprint/1900

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