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In vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxide-mediated process.

Udaya Sankar, K. and Deepan Raja, B. and Usha Rani, G. and Manohar, B. and Venkateswaran, G. (2007) In vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxide-mediated process. Nanomedicine : nanotechnology, biology, and medicine, 3 (4). pp. 273-80. ISSN 1549-9642

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Abstract

Nanotechnology in drug delivery is a rapidly expanding field. Nanosized liposomal preparations are already in use for efficient drug delivery with better therapeutic indices. Existing methods of liposome preparation are limited by problems of scale-up, difficulty in controlling size, and intercalation efficiency. Here we prepare amphotericin B-intercalated liposomes by a novel process where amphotericin B and purified phosphatidyl choline are solubilized in suitable solvent and precipitated in supercritical fluid carbon dioxide (known as a gas antisolvent technique), to obtain microsized particles that are subsequently introduced into a buffer solution. The morphology of liposomes was characterized through a phase-contrast microscope, and the particle size distribution studied by laser technique showed nanosize with a narrow range of size distribution (between 0.5 and 15 microm) and a higher intercalation efficiency. In vitro studies conducted using Aspergillus fumigatus (MTCC 870) strain proved to be efficient in the retardation of the growth of the organism.

Item Type: Article
Uncontrolled Keywords: Supercritical; Carbon dioxide; Gas antisolvent; Liposomes; Amphotericin B; In vitro studies
Subjects: 600 Technology > 02 Engineering & allied operations
Divisions: Food Engineering
Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 18 Aug 2008 11:36
Last Modified: 23 May 2012 07:10
URI: http://ir.cftri.com/id/eprint/1828

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