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Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes.

Umesh Hebbar, H. and Sumana, B. and Raghavarao, K. S. M. S. (2008) Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresource Technology, 99 (11). pp. 4896-902. ISSN 0960-8524

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Abstract

Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. The optimized conditions for the extraction from core resulted in forward and back extraction efficiencies of 45% and 62%, respectively, using reverse micellar system of cationic surfactant CTAB. A fairly good activity recovery (106%) and purification (5.2-fold) of bromelain is obtained under these conditions. Reverse micellar extraction from peel, extended stem and crown using CTAB system resulted in purification folds of 2.1, 3.5, and 1.7, respectively. Extraction from extended stem using anionic surfactant AOT in isooctane did not yield good results under the operating conditions employed.

Item Type: Article
Uncontrolled Keywords: AOT; Bromelain; CTAB; Pineapple waste; Reverse micellar extraction
Subjects: 600 Technology > 08 Food technology > 24 Fruits
600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Jun 2009 07:04
Last Modified: 28 Aug 2018 06:50
URI: http://ir.cftri.com/id/eprint/1827

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