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Isoflavone composition, phenol content, and antioxidant activity of soybean seeds from India and Bulgaria.

Sakthivelu, G. and Akitha Devi, M. K. and Giridhar, P and Rajasekaran, T and Ravishankar, G. A. and Nikolova, M T and Angelov, G B and Todorova, R M and Kosturkova, G P (2008) Isoflavone composition, phenol content, and antioxidant activity of soybean seeds from India and Bulgaria. Journal of Agricultural and Food Chemistry, 56 (6). pp. 2090-5. ISSN 0021-8561

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Abstract

Isoflavone levels and isoflavone chemical composition in 11 cultivars of soybean, including 4 Indian and 7 genotypes of soybean grown in Bulgaria, were analyzed as determined by C 18 reversed phase high-performance liquid chromatography coupled with a photodiode array detector. Antioxidant activity of soybean extracts was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and total phenolic compounds (TPC) were determined by using Folin-Ciocalteu reagent. The range of total isoflavones (TI) was 558.2-1048.6 microg g (-1) of soy in Indian cultivars, and it was 627.9-1716.9 microg g (-1) of soy in the case of Bulgarian cultivars. The highest and lowest total isoflavone contents were observed for Maus-2 (1048.6 microg g (-1) of soy) and Hardee (558.2 microg g (-1) of soy), respectively, for the Indian cultivars, and they were observed for Boryara (1716.9 microg g (-1) of soy) and Line 5 (627.9 microg g (-1) of soy) for the Bulgarian genotypes. DPPH radical scavenging activity did not differ significantly among the cultivars and did not correlate with TI, whereas TPC correlated well with TI and weakly with DPPH. Malonylglucoside of all the aglycones, total genistein (TGin), and total daidzein (TDin) showed strong correlation with total isoflavones, whereas acetylglucoside and aglycone levels did not significantly correlate with total isoflavone. Profiling of soybean isoflavone is helpful in understanding the regulation of isoflavone biosynthesis for greater improved resistance of crops to disease and greater health benefits for humans. This comparative study of soybean cultivars grown in India and Bulgaria throws light on their composition and nutraceutical value.

Item Type: Article
Uncontrolled Keywords: Soybean cultivars; isoflavones; antioxidant; HPLC
Subjects: 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Aug 2008 10:14
Last Modified: 29 Aug 2018 06:49
URI: http://ir.cftri.com/id/eprint/1808

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