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Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan.

Ayyappan, Appukuttan Aachary and Prapulla, S. G. (2008) Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan. Journal of Agricultural and Food Chemistry, 56 (11). pp. 3981-3988. ISSN 0021-8561

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Abstract

Eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (OSX) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (XOS) production. Aspergillus oryzae MTCC 5154, Aspergillus flavus , Aspergillus niger , and Aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of XOS from birch wood xylan (BWX). A. oryzae produced 89.5 +/- 1.13% XOS in the hydrolysate at 24 h of reaction. The effect of OSX, BWX, and raw corncob on the induction of endoxylanase in A. oryzae was studied, and the xylanase activity was maximum at 96 h of cultivation in 3% corncob containing medium. XOS produced at 36 h of reaction was 5.87 +/- 0.53 mg/mL (12 +/- 2% xylose, 48 +/- 2.43% xylobiose, and 40 +/- 3.6% higher oligomers) from 1% BWX . HPLC/refractive index detection and ESI/MS analysis of fractions obtained by GPC corresponded to neutral and 4- O-methyl-alpha- d-glucuronic acid substituted acidic oligosaccharides. The major fraction, beta- d-xylopyranosyl-(1-->4)- d-xylanopyranose was characterized using (13)C NMR.

Item Type: Article
Uncontrolled Keywords: Aspergillus oryzae MTCC 5154; endo-1,4-�-xylanase; corncob; xylooligosaccharides; xylobiose; 13C NMR
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Aug 2008 10:34
Last Modified: 21 Jun 2012 06:20
URI: http://ir.cftri.com/id/eprint/1785

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