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Spermine-Induced Morphogenesis and Effect of Partial Immersion System on the Shoot Cultures of Banana.

Venkatachalam, L. and Bhagyalakshmi, N. (2008) Spermine-Induced Morphogenesis and Effect of Partial Immersion System on the Shoot Cultures of Banana. Applied Biochemistry and Biotechnology, 151. pp. 502-511. ISSN 0273-2289

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Abstract

Contribution of exogenous polyamines (PAs) and polyamine-inhibitors on plantlet regeneration patterns of banana (cv. Nanjanagudu Rasabale-AAB) was studied and the performance of regenerated shoots in temporary immersion system was evaluated. The rhizome explants (without shoot bud) of in vitro shoots produced a mixture of embryogenic and nonembryogenic calli on modified MS medium. The analyses of endogenous pools of polyamines showed higher levels of PAs in embryogenic than in nonembryogenic calli. Supplementation of various levels of (10-50 muM) spermine (Spm), spermidine (Spd), and putrescine (Put) to cultures with secondary embryogenesis showed that about 50% of embryogenic calli rapidly produced secondary embryos only in the presence 40 muM Spm but not in other treatments. The crucial role of Spm was further confirmed by the use of 0.1 mM each of alpha-DL-Difluromethylornithine and alpha-DL-Difluromethylarginine along with Spm where the presence of inhibitors concomitantly inhibited the secondary embryogenesis. The shoots obtained from the embryogenic cultures were checked for their performance on solid medium (SM) and partial immersion system (PIS). The rate of shoot multiplication was higher in PIS than in SM throughout 6 weeks culture period. Uniformity in elongation of all the shoot buds was observed in PIS but not in SM. Evaluation for the acclimatization, survival under greenhouse conditions revealed the better performance of PIS-derived plants than those from SM.

Item Type: Article
Uncontrolled Keywords: Embryogenesis . Musa . Partial immersion system . Polyamine
Subjects: 600 Technology > 08 Food technology > 24 Fruits > 02 Banana
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jun 2011 06:12
Last Modified: 28 Dec 2011 09:34
URI: http://ir.cftri.com/id/eprint/1778

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