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Isolation of a free radical-scavenging antioxidant from water spinach (Ipomoea aquatica Forsk)

Nagendra Prasad, K and Soundar, Divakar and Gyarahally, R Shivamurthy and Aradhya, S. M. (2005) Isolation of a free radical-scavenging antioxidant from water spinach (Ipomoea aquatica Forsk). Journal of the Science of Food and Agriculture, 85. pp. 1461-1468.

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Abstract

<p align="justify">Ipomoea aquatica Forsk, a green leafy vegetable that is a rich source of vitamins and amino acids with many health benefits, has been explored for the isolation and identification of its bioactive compounds. Activity-guided repeated fractionation of a methanol extract on a silica gel column followed by an XAD column yielded a compound that exhibited antioxidant activity with an EC50 value of 83 ± 1.02 µgml−1 reaction mixture. It also showed very strong lipid peroxidation-inhibitory activity in a liposome model system with an EC50 value of 72.2 ± 0.9 µgml−1. However, it showed negligible metal-chelating activity. Based on UV, 2D nuclear magnetic resonance and gas chromatography/mass spectrometry studies, the compound was tentatively identified to be 7-O-β-D-glucopyranosyl-dihydroquercetin-3-O-α- D-glucopyranoside. This is the first report on the antioxidant properties of I aquatica leaf extracts.  2005 Society of Chemical Industry</p>

Item Type: Article
Uncontrolled Keywords: green leafy vegetable; Ipomoea aquatica Forsk; antioxidant activity; flavonol glycoside; 7-O-β-Dglucopyranosyl- dihydroquercetin-3-O-α-D-glucopyranoside
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 18 Flavonoid Chemistry
600 Technology > 08 Food technology > 23 Vegetables
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Sep 2005
Last Modified: 28 Dec 2011 09:25
URI: http://ir.cftri.com/id/eprint/177

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