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Investigation on the Production of Food Ingredients and Biotransformation Using Hairy Root Cultures

Suresh, B. (2003) Investigation on the Production of Food Ingredients and Biotransformation Using Hairy Root Cultures. PhD thesis, University of Mysore.

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Abstract

The study throws light on the production of betalaines from hairy roots of B. vulgaris and biotransformations using C. frutescens normal roots for the production of vanillin related metabolites. Various aspects viz. polyamines, MJ and down stream process methodologies have been studied to enhance the metabolite production. It was concluded that hairy roots can be used as a potential system for the production of betalaines. The organized nature and stable metabolite production makes them more amenable for bioreactor cultivation and scale-up studies. Among all bioreactors studied, acoustic mist bioreactor performed well with high growth and metabolite production. There was no mass transfer limitation observed in the acoustic mist bioreactor unlike others such as nutrient sprinkle and bubble column reactors. Osmolarity measurements proved reliable than conductivity for indirect estimation of biomass when the hairy roots were cultivated in bioreactor systems. 180 The studies on product enhancement (polyamines and MJ) and on-line extraction of pigment (using CTAB) provided an insight for integrating unit operations and developing a process for continuous operation and higher production of phytochemicals in bioreactor systems. It was found that the roots were still viable and showed normal growth even after CTAB treatment (0.002%) which would be an important observation to make this process a continuous one. This study has shown the production of DOPA and dopamine, which are very important pharmaceutical compounds from hairy roots of B. vulgaris. Further studies with tyrosine feeding led to higher production of DOPA and dopamine. This study is of importance in understanding the biosynthetic pathway of betalaines derived from DOPA and dopamine. Use of oxygenase inhibitors such as caffeic acid and catechin formed the basis to envisage the enzymatic pathways in betalaine production. The higher production of DOPA and dopamine under the influence of caffeic acid and catechin might be because of the inhibitory action of these chemicals on tyrosinase preventing further conversions.

Item Type: Thesis (PhD)
Uncontrolled Keywords: Plant cell cultures Secondary metabolites root cultures food ingredients biotransformation
Subjects: 600 Technology > 08 Food technology
600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 03 Dec 2007 11:24
Last Modified: 28 Dec 2011 09:31
URI: http://ir.cftri.com/id/eprint/1532

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