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Enhancement of nuclease P1 activity in low concentration of denaturants

Gangadhara, B.N. and Parigi Ramesh, Kumar and Prakash, V. (2008) Enhancement of nuclease P1 activity in low concentration of denaturants. Enzyme and Microbial Technology, 43. pp. 336-342.

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Abstract

Nuclease P1 is a zinc-dependent endonuclease from the mold Penicillium citrinum. Nuclease P1 exhibits an increase in activity in the presence of low concentrations of urea or GuHCI. At 0.05 M GuHCI or 1 M urea the enzyme activity enhanced maximally by 3- and 3.9-fold. The kinetic parameters indicate a decrease in Km with an increase in catalytic constant. The Km values for control and in presence of 0.05 M GuHCI or 1 M urea are 1.11 mg, 0.86 mg and 0.60 mg, respectively. In presence of metal ions such as Cu2+ and Co2+, urea or GuHCI treated enzyme still maintains the activity enhancement up to different extent. The far UV-CD results point to a minor conformational change and fluorescence spectra reveal increase in the relative fluorescence intensity at lower concentration. Thermal denaturation studies reveal increase in apparent Tm from 75 C for control to 80 C and 77 C in presence of 0.1 M GuHCI or 0.5 M urea, respectively. The above results show that the activation of nuclease PI in presence of low concentrations of denaturants is mainly because of the increase in the catalytic constant suggesting that activation is due to a more open and flexible conformation of the activated enzyme with increased catalytic efficiency.

Item Type: Article
Uncontrolled Keywords: Nuclease PI Urea GuHCI Enhanced enzyme activity Molten globule
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Oct 2007 11:44
Last Modified: 28 Dec 2011 09:31
URI: http://ir.cftri.com/id/eprint/1505

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