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Single Step Method of Preparation of Detergent- Permeabilized Kluyveromyces fragilis for Lactose Hydrolysis

Bachhawat, N. and Gowda, L. R. and Bhat, S. G. (1996) Single Step Method of Preparation of Detergent- Permeabilized Kluyveromyces fragilis for Lactose Hydrolysis. Process Biochemistry, 31 (1). pp. 21-25.

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Abstract

β-Galactosidase activity of CTAB-permeabilized K. fragilis depends upon the cell to detergent ratio and the pH of the permeabilizing medium. Maximum β-galactosidase activity was observed when the cell to detergent ratio was I: 001 at neutralpH. A simple and single step methodfor the preparation of whole cell β-galactosidase is described which involves adjusting the pH of the growth medium at the beginning of the stationary phase to 7.0, addition of CTA R directly to the growth medium at 1% concentration of biomass (wet weight), before harvesting cells by centrifugation. This whole cell enzyme activity readily hydrolysed high concentrations of lactose in buffer and whey.

Item Type: Article
Uncontrolled Keywords: Lactose K. fragilis Lactose hydrolysis
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast
500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 05 Carbohydrate Biochemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Oct 2007 11:09
Last Modified: 21 Jun 2012 06:26
URI: http://ir.cftri.com/id/eprint/1489

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