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Enhanced production of pediocin C20 by a native strain of Pediococcus acidilactici C20 in an optimized food-grade medium

Halami, Prakash M. and Chandrashekar, A. (2005) Enhanced production of pediocin C20 by a native strain of Pediococcus acidilactici C20 in an optimized food-grade medium. Process Biochemistry, 40. pp. 1835-1840.

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Abstract

The potentiality of a native isolate, Pediococcus acidilactici C20, to produce quantifiable amounts of pediocin C20 on whey permeate was studied. The molecular basis for the presence of a b-gal like gene was shown by DNA dot-blot technique followed by b-galactosidase (b-Gal) assay on native polyacrylamide gel as experimental evidence for lactose hydrolysis. Production of pediocin C20 was found to be 1–1.5-fold excess in lactose-based medium as compared to medium with glucose. Optimized whey permeate supplemented with 2% yeast extract gave cell growth of 3.5, OD600 and pediocin C20 activity of 150 103 AU ml�1 equivalent to that obtained by growth in commercial MRS broth.

Item Type: Article
Uncontrolled Keywords: Pediocin; Pediococcus acidilactici; b-Galactosidase; Whey permeate; Food-grade media
Subjects: 600 Technology > 08 Food technology > 05 Processing and Engineering
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Oct 2007 09:47
Last Modified: 28 Dec 2011 09:30
URI: http://ir.cftri.com/id/eprint/1481

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