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Mass transfer in a spray column during two-phase extraction of horseradish peroxidase

Srinivas, N. D. and Narayan, A. V. and Raghavarao, K. S. M. S. (2002) Mass transfer in a spray column during two-phase extraction of horseradish peroxidase. Process Biochemistry, 38. pp. 387-391.

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Multistage contactors are often needed to achieve the desired degree of mass transfer. One such contactor is spray column, which can be conveniently used to isolate and purify proteins using aqueous two-phase extraction. The effects of orifice size and addition of NaCl on the overall mass transfer coefficient (KLa) of horseradish peroxidase (HRP) and fractional dispersed phase hold up (oD)have been studied in a simple spray column using a polyethylene glycol/potassium phosphate aqueous two-phase system. KLa and oD increased with an increase in dispersed phase superficial velocity (vs) as well as capillary diameter. The presence of NaCl increased KLa. The overall mass transfer coefficient for HRP normalized for dispersed phase hold up, was found to be independent of vs in the range studied.

Item Type: Article
Uncontrolled Keywords: Horseradish peroxidase; Mass transfer coefficient; Hold up; Aqueous two-phase systems; Spray column
Subjects: 600 Technology > 08 Food technology > 07 Food Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Oct 2007 07:35
Last Modified: 03 Oct 2018 09:20
URI: http://ir.cftri.com/id/eprint/1473

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