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Enzymic synthesis of isoamyl isovalerate using immobilized lipase from Rhizomucor miehei: a multivariate analysis

Chowdary, G. V. and Ramesh, M. N. and Prapulla, S. G. (2000) Enzymic synthesis of isoamyl isovalerate using immobilized lipase from Rhizomucor miehei: a multivariate analysis. Process Biochemistry, 36. pp. 331-339.

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Abstract

Isoamyl isovalerate, an ester with apple flavour was synthesized using Rhizomucor miehei lipase immobilized on a weak anion-exchange resin (Lipozyme IM-20) by the direct esterification of isoamyl alcohol and isovaleric acid. Various reaction parameters including substrate concentration, enzyme concentration, substrate molar ratio, temperature and incubation time affecting the synthesis of isoamyl isovalerate were investigated. A maximum yield of \85% was obtained at 0.5 M (acid) substrate concentration (1.5:1 alcohol:acid ratio) using 10 g:l enzyme concentration and a reaction temperature of 50°C for 144 h incubation time in n-hexane. A solvent screening study indicated that hydrophobic solvents including cyclohexane, hexane, heptane:isooctane are the most suitable ones for esterification. The feasibility of synthesizing the ester under solvent-free conditions has also been studied. However, the conversions were very low. The parameters influencing the yield were modeled using multivariate analysis. These equations can be applied to predict the yield at any other known values of the input parameter.

Item Type: Article
Uncontrolled Keywords: Esterification; Isoamyl isovalerate; Rhizomucor miehei lipase; Lipozyme IM-20; Organic solvent; Modeling
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 24 Organic Chemistry
600 Technology > 08 Food technology > 15 Flavour/Fragrance/Perfumes
Divisions: Fermentation Technology and Bioengineering
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 01 Oct 2007 13:03
Last Modified: 30 Dec 2016 11:13
URI: http://ir.cftri.com/id/eprint/1460

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