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On-line monitoring of citric acid production in solid-state culture by respirometry

Pintado, J. and Lonsane, B. K. and Gaime Perrau, I. and Roussos, S. (1998) On-line monitoring of citric acid production in solid-state culture by respirometry. Process Biochemistry, 33 (5). pp. 513-518.

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Abstract

The aim of this work was to study the possibility of monitoring citric acid production from mussel processing wastes (MPW) by Aspergillus niger in solid-state culture (SSC) on an inert support. This was conducted by measuring CO2 and 02 concentration in exhaust gases, using an automatic sampler connected to a gas chromatograph and a data acquisition system. The procedure permitted information on the physiological state of the culture to be obtained. A relationship between citric acid accumulation and a decreasc in CO, production was found, allowing citric acid production to be followed in real-time. Moreover, respiratory activity (p,.) can be estimated and the effect of different variables on this parameter studied. Initial nitrogen concentration, a critical factor for achieving high production of citric acid from MPW in submerged culture revealed no effect in SSC. This indicates a tolerance of SSC to higher concentrations of nitrogen con- stituting an advantage when using residual media with high levels of protein and wlriability in their composition.

Item Type: Article
Uncontrolled Keywords: solid-state culture, respirometry, citric acid, mussel processing wastes aspergillus niger
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 08 Food technology > 28 Meat, Fish & Poultry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 27 Sep 2007 11:32
Last Modified: 13 Jun 2012 11:25
URI: http://ir.cftri.com/id/eprint/1449

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