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Bifdobacterium adolescentis is intrinsically resistant to antitubercular drugs.

Dhanashree, L. and Raman, P. and Rajagopal, Kammara (2018) Bifdobacterium adolescentis is intrinsically resistant to antitubercular drugs. Scientific Reports, 8. pp. 1-15.

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Abstract

Multiple mutations in the β subunit of the RNA polymerase (rpoβ) of Mycobacterium tuberculosis (Mtb) are the primary cause of resistance to rifamycin (RIF). In the present study, bifdobacterial rpoβ sequences were analyzed to characterize the mutations that contribute to the development of intrinsic resistance to RIF, isoniazid, streptomycin and pyrazinamide. Sequence variations, which mapped to cassettes 1 and 2 of the rpoβ pocket, are also found in multidrug-resistant Mtb (MDR Mtb). Growth curves in the presence of osmolytes and diferent concentrations of RIF showed that the bacteria adapted rapidly by shortening the growth curve lag time. Insight into the adapted rpoβ DNA sequences revealed that B. adolescentis harbored mutations both in the RIF pocket and in regions outside the pocket. The minimum inhibitory concentrations (MICs) and mutant prevention concentrations (MPCs) indicated that B. longum, B. adolescentis and B. animalis are resistant to antitubercular drugs. 3D-homology modeling and binding interaction studies using computational docking suggested that mutants had reduced binding afnity towards RIF. RIF-exposed/resistant bacteria exhibited variant protein profles along with morphological diferences, such as elongated and branched cells, surface conversion from rough to smooth, and formation of a concentrating ring.

Item Type: Article
Uncontrolled Keywords: Bifdobacterium adolescentis, resistant bacteria, intrinsic resistance
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Aug 2020 10:38
Last Modified: 19 Aug 2020 10:38
URI: http://ir.cftri.com/id/eprint/14462

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