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In vitro multiplication of Vanilla planifolia using axillary bud explants

George, P. S. and Ravishankar, G. A. (1997) In vitro multiplication of Vanilla planifolia using axillary bud explants. Plant Cell Reports, 16. pp. 490-494.

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Abstract

A clonal propagation method has been developed for efficient multiplication of Vanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l–1) and a-naphthaleneacetic acid (NAA, 1 mg l–1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l–1 and NAA at 0.5 mg l–1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots in V. planifolia.

Item Type: Article
Uncontrolled Keywords: Vanilla planifolia Axillary bud Liquid media Multiple shoots Plantlets
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Sep 2007 11:50
Last Modified: 28 Dec 2011 09:30
URI: http://ir.cftri.com/id/eprint/1431

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