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Synergistic method for extraction of high purity Allophycocyanin from dry biomass of Arthrospira platensis and utilization of spent biomass for recovery of carotenoids.

Hrishikesh, A. Tavanandi and Prasad, V. and Raghavarao, K. S. M. S. (2019) Synergistic method for extraction of high purity Allophycocyanin from dry biomass of Arthrospira platensis and utilization of spent biomass for recovery of carotenoids. Separation and Purification Technology, 225. pp. 97-111. ISSN 1383-5866

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Abstract

Allophycocyanin (A-PC) and carotenoids are high value low volume compounds present in Arthrospira platensis which are usually ignored and left un-extracted (50- 80%) in the spent biomass after extraction of C-Phycocyanin. In the present work, process parameters such as incubation time, solid-liquid (S/L) ratio and surfactant/enzyme concentration was standardized for surfactant and enzyme-assisted extraction of A-PC. Enzyme-assisted method resulted in very high A-PC purity (1.19) with a yield of 32.27 mg/g dry biomass (db). To improve the yield, enzyme-assisted method was combined with ultrasonication which resulted in the highest yield of 44.08 mg/g (db) with a purity of 1.09 (the highest reported till date during primary extraction). The spent biomass after Low-Humidity (LH) drying was used for the extraction of carotenoids and the best conditions were 80% concentration of ethanol, 1:4 S/L ratio, pH 7, 40 oC temperature and 1 h extraction time. LH drying was observed to be a possible alternative to freeze drying for drying of spent biomass.

Item Type: Article
Uncontrolled Keywords: Enzyme-assisted extraction; Surfactant; Ultrasonication; Phycobiliproteins; Value addition
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Jun 2019 09:12
Last Modified: 04 Jun 2019 09:12
URI: http://ir.cftri.com/id/eprint/14134

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