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Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development.

Kiran, K. and Priyanka, P. S. and Veeresh, Lokesh and Nandini, P. Shetty and Giridhar, P. (2018) Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development. 3 Biotech, 8. pp. 1-11.

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Abstract

To explore the transcriptional control of phenylpropanoid pathway (PPP) involved in vanillin flavour metabolites production in tuberous roots of Decalepis hamiltonii, four PPP key genes expressed during the tuber development were identified and their mRNA expression profiles were evaluated using quantitative real-time PCR. Flavour metabolite quantification by HPLC analysis confirmed 10, 170 and 500 μg/g 2-hydroxy-4-methoxy benzaldehyde and 4, 20 and 40 μg/g vanillin in first- (3-monthold plant), second- (18-month-old plant) and third-stage tubers (60-month-old matured plant), respectively. The expression of all four genes phenylalanine ammonia lyase (DhPAL), cinnamate-4-hydroxylase (DhC4H), caffeic acid-O-methyltransferase (DhCOMT) and vanillin synthase (DhVAN) increased with flavour development from first stage to second stage. A decrease in expression from 1.9-folds to 0.1-folds and 19.2-folds to 5.2-folds for DhCOMT and DhVAN was recorded for second stage to third stage, respectively. However, a gradual increase in expression of DhPAL (up to 26.4-folds) and a constant expression pattern for DhC4H (up to 7.1-folds) was evident from second stage to third stage of flavour development. The decrease in the expression levels of DhCOMT and DhVAN in third stage shows that the second-stage tubers are more transcriptionally active towards flavour biosynthesis.

Item Type: Article
Uncontrolled Keywords: 2-Hydroxy-4-methoxy benzaldehyde · Phenylalanine ammonia lyase · Cinnamate-4-hydroxylase · Caffeic acid- O-methyl transferase · Vanillin synthase · Relative expression
Subjects: 600 Technology > 08 Food technology > 15 Flavour/Fragrance/Perfumes
600 Technology > 08 Food technology > 23 Vegetables
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 31 May 2019 03:57
Last Modified: 31 May 2019 03:57
URI: http://ir.cftri.com/id/eprint/14092

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