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Immunomodulatory activity of non starch polysaccharides isolated from green gram (Vigna radiata).

Kiranmayi, K. and Muralikrishna, G. (2018) Immunomodulatory activity of non starch polysaccharides isolated from green gram (Vigna radiata). Food Research International, 113. pp. 269-276. ISSN 0963-9969

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Abstract

Green gram, rich in dietary fiber is known to enhance the function of immune system. However information pertaining to the immunomodulatory potential of its non starch polysaccharides (NSPs) is scanty. Hence, five different NSPs were extracted successively using water (WSP), hot water (55 o C, HWSP), EDTA (0.5%, Pectins) and alkali (10%, Hemicellulose A and B) which varied in their arabinose to galactose ratio, sugar, protein, uronic acid contents, molecular weight distribution and immunomodulatory activity. Hemicellulose B was relatively rich in carbohydrate content (~95%) and also possessed potent immunomodulatory activity among the various NSPs. Hemicellulose B was further fractionated on DEAE-cellulose column into six different fractions by eluting step-wise with water, ammonium carbonate (0.1, 0.2, 0.3MAC) and sodium hydroxide (0.1 and 0.2M NaOH). 0.1MAC eluted fraction was found to be the major one amounting to ~ 50% yield and showed relatively significant (p < 0.001) activity towards splenocyte proliferation and macrophage activation as compared with rest of the DEAE eluted fractions.

Item Type: Article
Uncontrolled Keywords: Green gram Non starch polysaccharides Immunomodulation Splenocyte proliferation Macrophage activation Ion exchange chromatography
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 06 Feb 2019 04:36
Last Modified: 06 Feb 2019 04:36
URI: http://ir.cftri.com/id/eprint/13926

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