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β-carotene isolated from the marine red alga, Gracillaria sp. potently attenuates the growth of human hepatocellular carcinoma (HepG2) cells by modulating multiple molecular pathways

Yogendra Prasad, K. and Deepika, U. R. and Sowya Shree, G. and Arpitha, H. S. and Nimish Mol, Stephen and Rangaiah, K. and Ganesan, P. (2019) β-carotene isolated from the marine red alga, Gracillaria sp. potently attenuates the growth of human hepatocellular carcinoma (HepG2) cells by modulating multiple molecular pathways. Journal of Functional Foods, 52. pp. 165-176. ISSN 1756-4646

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Abstract

There remains a remarkable controversy on the anti-cancer stand of β-carotene specifically when high concentrations are used. This study aimed to examine the growth inhibitory effect of the lower concentrations of β- carotene in HepG2 cells. Beta-carotene purified from Gracillaria sp. inhibited viability of HepG2 cells in a concentration-dependent manner. This growth inhibitory effect was well correlated with increased apoptotic cell death. Apoptosis induction by β-carotene was associated with increased caspase-3 activity and reduced mitochondrial membrane potential. Western blot analysis showed a significant decrease in the expression of Bcl-2, PARP, and NF-kB protein, and an increase in the protein expression of Bax. Beta-carotene also blocked the activation of intracellular growth signaling proteins, Akt and ERK1/2. Further, it down-regulated the expression of endogenous antioxidant enzymes, SOD-2 and HO-1, and its transactivation factor, Nrf-2. The current study emphasizes the role of β-carotene as an effective inhibitor of the growth of hepatocarcinoma cell.

Item Type: Article
Uncontrolled Keywords: Gracillaria sp. Hepatocarcinoma cells Beta-carotene Apoptosis Cell survival Redox markers
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions: Food Safety Analytical Quality Control Lab
Molecular Nutrition
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Feb 2019 05:49
Last Modified: 04 Feb 2019 05:49
URI: http://ir.cftri.com/id/eprint/13908

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