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Polymeric chitosan-glycolipid nanocarriers for an effective delivery of marine carotenoid fucoxanthin for induction of apoptosis in human colon cancer cells (Caco-2 cells).

Ravi, Hindupur. and Kurrey, N. K. and Yuki, Manabe and Tatsuya, Sugawara and Baskaran, V. (2018) Polymeric chitosan-glycolipid nanocarriers for an effective delivery of marine carotenoid fucoxanthin for induction of apoptosis in human colon cancer cells (Caco-2 cells). Materials Science and Engineering: C, 91. pp. 785-795.

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Abstract

Fucoxanthin (FUCO), a marine carotenoid is photo-, and thermo-labile and poorly bioavailable due to its lipophilicity. Hence, we developed a chitosan (CS) + glycolipid (GL) nanogels (NGs) to increase cellular uptake and anticancer efficacy of FUCO (10 μM) in human colon cells (Caco-2). Effect of FUCO loaded in NGs with/with no GL was studied in comparison with micellar FUCO. Results showed that the cell viability was lower (p < 0.05) in NGs + GL (50.5%) compared to NGs (-GL) (66.5%) and the mixed micelles (72.5%) groups over 48 h exposure. An enhanced reactive oxygen species (ROS) generation was evident in NGs + GL (379.2%) group compared to NGs (−GL) and mixed micelles groups. Further, induction of apoptosis with an increased chromatin condensation and DNA fragmentation as evidenced in DAPI staining and DNA ladder assay were higher in NGs + GL group than other groups. Down-regulation of Bcl-2 (6.6 folds) was higher in NGs + GL group compared to NGs (−GL) (1.94 fold) and mixed micelles (1.19 fold) groups. Higher Bax up-regulation in NGs + GL compared to other groups supports the Bcl-2 down regulation. Mitochondrial membrane polarisation (ΔΨm) was higher in NGs + GL group (2.46 fold) compared to NGs (−GL) (1.91 fold) and mixed micelles (1.26 fold) groups. The cellular FUCO uptake illustrated a positive correlation between its level (pmol/106 cells) in NGs + GL (758.3) and enhanced caspase-3 activity (25.8 folds). This could be the reason for an increased apoptotic activity in NGs + GL group than other groups. Results demonstrate that delivery of FUCO in NGs + GL carrier aids cellular uptake and chemotherapeutic potential of FUCO. Results further demonstrate, for the first time, higher anti-cancer activity of FUCO loaded in NGs + GL and the effect was through ROS generation via a caspase dependent mechanism in Caco-2 cells.

Item Type: Article
Uncontrolled Keywords: Fucoxanthin Chitosan Glycolipid Apoptosis Nanocarrier Caco-2
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 03 Sep 2018 06:52
Last Modified: 03 Sep 2018 06:52
URI: http://ir.cftri.com/id/eprint/13839

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