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Optimization of polyhydroxybutyrate production by Bacillus sp.CFR 256 with corn steep liquor as a nitrogen source

Vijayendra, S. V. N. and Rastogi, N. K. and Shamala, T. R. and Anil Kumar, P. K. (2007) Optimization of polyhydroxybutyrate production by Bacillus sp.CFR 256 with corn steep liquor as a nitrogen source. Indian Journal of Microbiology, 47. pp. 170-175.

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Abstract

Polyhydroxyalkanotes (PHAs), the eco-friendly biopolymers produced by many bacteria, are gaining importance in curtailing the environmental pollution by replacing the nonbiodegradable plastics derived from petroleum. The present study was carried out to economize the polyhydroxybutyrate (PHB) production by optimizing the fermentation medium using corn steep liquor (CSL), a by-product of starch processing industry, as a cheap nitrogen source, by Bacillus sp. CFR 256. Response surface methodology (RSM) was used to optimize the fermentation medium using the variables such as corn steep liquor (5-25 g l-1), Na2HP04 2H20 (2.2-6.2 g 1-1), KH2P04 (0.5-2.5 g H), sucrose (5-55 g H) and inoculum concentration (1-25ml H). Central composite rotatable design (CCRD) experiments were carried out to study the complex interactions of the variables. The optimum conditions for maximum PHB production were (g H): CSL - 25, Na2HP04 2H20 - 2.2, KH2P04 - 0.5, sucrose - 55 and inoculum - 10 (ml H). After 72 h of fermentation, the amount of PHA produced was 8.20 g 1-' (51.20 % of dry cell biomass). It is the first report on optimization of fermentation medium using CSL as a nitrogen source, for PHB production by Bacillus sp.

Item Type: Article
Uncontrolled Keywords: Bacillus Corn steep liquor Central composite rotatable design Optimization Polyhydroxybutyrate Response surface methodology
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 27 Polymer Chemistry
600 Technology > 07 Beverage Technology > 01 Alcoholic beverage
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 31 Jul 2007 04:01
Last Modified: 30 Jun 2015 12:23
URI: http://ir.cftri.com/id/eprint/1375

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