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Kinetic Studies on Transfructosylation of Sucrose by Fructosyltransferase

Smitha Rao, Y.G (2007) Kinetic Studies on Transfructosylation of Sucrose by Fructosyltransferase. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: In the present investigation, kinetics of transfructosylation reaction by FTase from Aspergillus oryzae MTCC 5154 was studied. Crude enzyme was used for the production of FOS at lab, bench and pilot scale and hence, crude enzyme has been used for the determination of kinetic parameters. The enzyme was produced by submerged fermentation and the crude enzyme was used for the kinetic studies. Kinetic parameters (kcat, Km and Ki) were determined from the experimental data at various substrate concentrations and also in the presence of glucose. Transfructosylation was inhibited by glucose by mixed inhibition (Ki d-glucose = 0.8M). Time course of FOS indicated that percentage of FOS had reached a maximum of 28% by the eighth hour of incubation.
Uncontrolled Keywords: Fructooligosaccharides transfructosylation Aspergillus niger kinetic parameters
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 27 Jul 2007 04:41
Last Modified: 28 Dec 2011 09:29
URI: http://ir.cftri.com/id/eprint/1364

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